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Increased endometrial cytokine synthesis and inflammation in PCOS patients induced by excess androgens through TLR4/IRF-7/NF-B signaling contributes to inflammation in PCOS patients (143)

Both are complementary in multi-tissue protocols, with non-redundant mechanisms

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To confirm whether recombinant proteins of AsGGT1, AsGGT2, and AsGGT3 were expressed as mature GGT enzymes in yeast cells, we first examined deglutamylation activities of these recombinant proteins by using the standard procedure that utilizes -glutamyl- p -nitroanilide, a common synthetic -glutamyl donor substrate for known GGTs (Orlowski and Meister, 1963)