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Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis
The general obesity population in TRIUMPH-1 showed dysesthesia at a lower rate than TRIUMPH-4's 20.9%, which is consistent with differences between the two populations (TRIUMPH-4's cohort was older and had more comorbidities)
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