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paraffin embedded (FFPE) tissue samples and sections
Resistance to ampicillin is conferred by the bla gene which encodes β-lactamase that cleaves the β-lactam bond of the antibiotic
and may be used in cold rooms or incubators
Small RNAs (e
5-Fluoroorotic Acid (5-FOA) Purification paraffin embedded (FFPE) tissue samplesUsing 5 Fluoroorotic Acid (5 FOA) for the counter selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two hybrid screens are methods that can employ the use of 5 FOA. Otherwise nontoxic to yeast, 5 FOA is converted to the toxic form (i. e., 5 flurouracil) in strains expressing the functional URA3 gene coding for orotine 5 monophosphate decarboxylase that is involved in
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