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This stack is supplied solely as research reagents for qualified professionals conducting in vitro or animal-model studies

Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)

During this time: Use reliable birth control Track your menstrual cycles Take prenatal vitamins with folic acid Focus on healthy eating habits Step 3: Wait for Your Body to Heal (If Needed) If you just had a miscarriage, wait for at least one normal period