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For total DNA staining, membranes were incubated in 0.1% (wt/vol) methylene blue solution containing 0.5 M sodium acetate (pH 5.2) overnight at room temperature

10.1111/j.1749-6632.2000.tb05456.x 68

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423 Furthermore, the study emphasized that neurons in specific brain regions, such as the hindbrain and hypothalamus, co-express GLP-1R along with 1-adrenergic and -opioid receptors, suggesting a localized mechanism within the CNS that could coordinate peripheral anti-inflammatory responses