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The antiallodynic effect was long-lasting, with a peak effect at 1 h post-gavage and a duration exceeding 4 h [71]

Lab method Liquid chromatography-tandem mass spectrometry (LC-MS/MS) or high-quality immunoassays are preferred for accuracy

Monitoring the effluent at 220 nm, chromatography (flow rate: 1.0 mL/min) was started with a mixture of H 2 O/MeOH (40/60, v/v), the MeOH content was kept constant for 1 min at 60%, increased within 20 min to 80%, increased within 5 min to 86%, increased within 10 min to 90%, increased within 1 min to 100%, kept constant for 3 min, decreased within 1 min to 60% MeOH, followed by re-equilibration for 4 min