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Using brain imaging, authors have shown that patterns of brain activity expressed during training on a serial reaction time motor task reappear during subsequent REM sleep [26], Functional Magnetic Resonance Imaging (fMRI) has also been used to compare patterns of brain activation before and after a night of sleep

In published mouse studies, 50 mg/kg IP twice daily for 28 days is the highest-dose, longest-duration regimen reported in peer-reviewed literature

Better still, if you know you will be on 0.25mg for four weeks and then 0.5mg for the next four weeks, you can plan your reconstitution date so that the 28-day window covers both titration steps

Reference: Kanbay, M., Copur, S., Siriopol, D., Yildiz, A