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The plates were incubated for 24 h at 37C, followed by centrifugation at 2,000 rpm for 5 min, and 50 l of supernatant was transferred to a fresh flat-bottom microplate (BD Falcon )

FIGURE 7 Discussion In animal experiments, we verified the therapeutic effect of berberine on T2DM and the function improvement of islet cell

Nat Med (2020) 26(3):32632

Trong , mt s thnh phn quan trng c tc dng: Glutathione : Glutathione gip trung ha cc gc t do, bo v t bo da khi tn thng v c ch s hnh thnh melanin, gip da sng mn, gim nm