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muciniphila cultured in a medium supplemented with glucose, GlcNAc, soy peptone, and threonine had the same growth efficiency as that in a mucus-based medium (Plovier et al., 2017)

Pinealon: 100 to 300 mcg subcutaneously daily for 10 to 20 days

Participants ingested a standardized commercially available liquid meal (Fresubin Energy Drink, 200 mL, 300 kcal [50E% carbohydrate, 15E% protein and 35E% fat]

The interview schedule was developed through discussion with healthcare professionals including a clinician working in a tier 3 obesity service, a diabetes physician, and a psychiatrist specialising in diabetes and mental health, as well as a member of the public with lived experience of taking a GLP-1 receptor agonist (see supplementary material p3)