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No studies have directly evaluated the cardiovascular effects of correcting vitamin B 12 deficiency in patients with known cardiovascular disease, although numerous studies have failed to demonstrate that correction of hyperhomocysteinemia itself reduces cardiovascular mortality or cardiovascular complications

B12 deficiency causes fatigue, anemia, and neurologic symptoms, and is more common in older adults, vegans, and those with malabsorption conditions

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The cells were then incubated in a humidified chamber at RT for 1 hour in primary antibodies diluted in 2.5 % BSA and 0.1 % Triton in PBS