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Hopkins's initial characterisation of the molecule, published in 1921 and refined in subsequent papers, described it as a dipeptide containing cysteine and glutamic acid

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[168], he proposed that NO may bind to the enzymes of mitochondrial ETC, such as NADH succinate oxidoreductase, NADH ubiquinone oxidoreductase, and cis aconitase and induce uncoupling of OXPHOS followed by the inhibition of glycolysis [66] and depletion of ATP in the cells [169]

Furthermore, most professional medical insurance providers in the UK wont cover practitioners using unlicensed products, leaving patients vulnerable if a complication occurs